Coding

Part:BBa_K385004:Design

Designed by: Krystal Annand, Joseph Hoare, Stephen Lam, Justyna Kucia   Group: iGEM10_Aberdeen_Scotland   (2010-09-27)


Phage lambda N-peptide, tandem repeat


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 29
    Illegal SapI.rc site found at 116


Design Notes

The part was engineered with an AUG, but no stop codon, to allow the part to be used as a translational fusion with another downstream open reading frame. A glycine rich spacer peptide was inserted at the 3' end of each of the tandem N-peptide repeats, to allow the N-peptide to be separated from each other, and any downstream ORF by a flexible linker. (Linker sequence GGT GAC GGT GCT GGT TTA ATT AAC)


Source

Phage lambda genome

References